Hi Qiime 2 Forum, I recently got another issue with running a command line which is: qiime dada2 denoise-single --i-demultiplexed-seqs manifest.qza --p-trunc-len 0 --output-dir dada2 --p-n-threads 12 --verbose I have merged reads from Geneious imported into Qiime 2 and running it, I passed this c…

Hi @Sammi , You are correct that you shouldn't be passing merged reads into DADA2. This is because DADA2 has its own internal merging process. One of the important issues with feeding the pre-merged reads is that quality scores in those merging regions change, and depending on the tool you are using…

Thank you Mehrbod_Estaki! I am currently running the subset of my data and again having troubleshooting with this msg..I am at DADA2 phase to de-noise my samples but I am unsure why I am encountering this error? Something to do with my computer setting? (qiime2-2019.7) qiime2@qiime2core2019-7:~/D…

Hi @Sammi , This is a brand new error, does that mean you followed my earlier recommendation and re-imported unmerged fastq files? It would be good to clarify these. As for the Kill Signal error here, I'm not too sure but I wonder if you are running out of memory. How much memory do you have alloca…

First, i did followed your earlier recommendation and re-imported unmerged fastq files. Second, When i set the qiime 2 environment, i think i followed through the protocol on the Qiime 2 virtual box install protocol. I did exactly the same..

Hi @Sammi , [image] Sammi: First, i did followed your earlier recommendation and re-imported unmerged fastq files. Ok! Thanks for clarifying. [image] Sammi: i followed through the protocol on the Qiime 2 virtual box install protocol. I did exactly the same… While that tutorial does ge…

Thank you so much!! I will definitely try that and hope for no more errors!

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Error on dada2 denoising

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