I am trying to demultiplex the multiplexed.qza file I created while importing my data. I keep getting the error “Error: no such option: --m-barcodes-file”. What am I doing wrong? I am running the current version of QIIME2 (2018.2). Here is my code thus far:
Hi Colin,
Thank you, I guess I have a couple questions:
Do I need to upload separate files for the forward and reverse reads?
Was this a change that was implemented when the version updated? In the qiime2 documentation, it only uses --m-barcodes-file…if so, it would be helpful if this was updated in the tutorials
Nope, the method only supports demultiplexing reads with the barcode in the forward read.
No - this has always been the name of this parameter.
It looks like you are confusing the parameters in the two demux methods in the cutadapt plugin --- the cutadapt demux-single method uses --m-barcodes-file, while cutadapt demux-paired uses --m-forward-barcodes-file. This is deliberate, because in the future we plan to support some form or forms of dual-index demux of paired-end reads - this could potentially require a reverse-barcodes parameter, so we are preemptively naming the forward parameter explicitly. Make sense?