Hello @Eman, based on that tutorial, and the initial import command you posted, you have imported your data as Phred33V2. The error is suggesting this may be the incorrect offset here in the tutorial it indicates the other options for importing. Perhaps you can try Phred64 or Phred64V2? To be honest with you, I am not sure which is correct for your data! (Also, that tutorial was not created by the QIIME 2 team, so I am not familiar with it, but it does seem perfectly suitable at a glance).