# error message from deblur
**URL:** https://forum.qiime2.org/t/error-message-from-deblur/12686
**Category:** User Support
**Created:** [December 1, 2019, 2:10pm UTC](https://forum.qiime2.org/t/error-message-from-deblur/12686 "2019-12-01T14:10:57Z")
**Posts on this page:** 4
**Page:** 1
### Author:  [@SciCatalyst](https://forum.qiime2.org/u/SciCatalyst)
#### Post date: [December 1, 2019, 2:10pm UTC](https://forum.qiime2.org/t/error-message-from-deblur/12686/1 "2019-12-01T14:10:57Z")
Hi Team,
I have been trying to do the denoising of my data using the deblur. Following the tutorial ''[Alternative methods of read-joining in QIIME 2 — QIIME 2 2019.1.0 documentation](https://docs.qiime2.org/2019.1/tutorials/read-joining/)'', i have joined and filtered the paired end reads, but deblur denoise plugin is giving me the error below:
**Plugin error from deblur:**
Command '['deblur', 'workflow', '--seqs-fp', '/tmp/qiime2-archive-ppi4z3cm/01ce9654-3f1b-4288-a0cb-ec86f3b857ba/data', '--output-dir', '/tmp/tmpp0x7\_2p2', '--mean-error', '0.005', '--indel-prob', '0.01', '--indel-max', '3', '--trim-length', '435', '--left-trim-length', '0', '--min-reads', '10', '--min-size', '2', '--jobs-to-start', '1', '-w', '--keep-tmp-files']' returned non-zero exit status 1.
**Command used:**
qiime deblur denoise-16S --i-demultiplexed-seqs home/qiime2/Desktop/demux-joined-filtered.qza --p-trim-length 435 --p-sample-stats --o-representative-sequences home/qiime2/Desktop/rep-seqs.qza --o-table home/qiime2/Desktop/table.qza --o-stats home/qiime2/Desktop/deblur-stats.qza
_The below information is for your reference:_
**For trim length :** No matter what length I give, it throws similar error.
**This is the interactive plot information and overview of the demultiplexed filtered count:**
| Minimum: | 266 |
| --- | --- |
| Median: | 58303.5 |
| Mean: | 59164.08 |
| Maximum: | 83565 |
| Total: | 2958204 |
**These are the steps that I have done:**
**raw reads to demux input file with manifest file (prepared)**
used input format PairedEndFastqManifestPhred33V2(as Phread64V2 gives error,)
**Joining Reads using vsearch**
**visualizing the summary of demux data**
**Sequence quality control**
**visualizing summary of filtered demux data**
Doubts:
Could this be possible as I have used raw data **assuming** that the primers and adaptors have already been removed from the reads? The files reads with extention: \*L001\_R001.fastq.gz. (as I don't have access to the indices used for the sequencing)
Best
Ojasvi
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### Author:  [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [December 4, 2019, 4:24pm UTC](https://forum.qiime2.org/t/error-message-from-deblur/12686/2 "2019-12-04T16:24:36Z")
Hi @SciCatalyst,
Could you please run this command with the `--verbose` flag to view the complete error message?
Thanks.
---
### Author:  [@SciCatalyst](https://forum.qiime2.org/u/SciCatalyst)
#### Post date: [December 5, 2019, 6:15pm UTC](https://forum.qiime2.org/t/error-message-from-deblur/12686/3 "2019-12-05T18:15:55Z")
Dear Bokulich,
Greetings.
I would like to inform that the problem has been resolved.
Actually I am running QIIME on Ubuntu in Oracle Virtual Box and was suggested that allocation of memory may be a problem, so just changed it and it worked. EUREKAAA!!!!!!!
Thank you.
Best,
Ojasvi
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [January 6, 2020, 12:15am UTC](https://forum.qiime2.org/t/error-message-from-deblur/12686/4 "2020-01-06T00:15:59Z")
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