Error ManifestFile Import FastQ PairEndSequenceWithQuality

Hi Team!!

I’m doing some 16S18S analysis, I’ve been successful several times. But I’m having a problem importing a ManifestFile from a new sample. Could you help me identify the error.

> qiime tools import --type 'SampleData[PairedEndSequencesWithQuality]' --input-path ManifestFile --output-path sampleimport.qza --input-format PairedEndFastqManifestPhred33

Traceback (most recent call last):
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/q2cli/tools.py”, line 146, in import_data
view_type=input_format)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/result.py”, line 240, in import_data
return cls.from_view(type, view, view_type, provenance_capture)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/result.py”, line 265, in _from_view
result = transformation(view)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/core/transform.py”, line 70, in transformation
new_view = transformer(view)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_types/per_sample_sequences/_transformer.py”, line 338, in _8
single_end=False)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_types/per_sample_sequences/_transformer.py”, line 268, in _fastq_manifest_helper
absolute=True)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_types/per_sample_sequences/_transformer.py”, line 158, in _parse_and_validate_manifest
_validate_paired_end_fastq_manifest_directions(manifest)
File “/home/robert/raid_robert/conda/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_types/per_sample_sequences/_transformer.py”, line 211, in _validate_paired_end_fastq_manifest_directions
‘“reverse”, but observed: %s’ % direction)
ValueError: Directions can only be “forward” or “reverse”, but observed: reverse

An unexpected error has occurred:

Directions can only be “forward” or “reverse”, but observed: reverse

See above for debug info.


Hi @Engo_Rafael_Gustavo, what version of QIIME 2 are you using? This format (PairedEndFastqManifestPhred33) was superceded in the 2019.4 release by a new, improved manifest format. If you aren’t running 2019.4, please upgrade to that and follow the guide for creating a manifest file. Thanks!

1 Like

Thank´s! My version is 2019.1! I will!