I have one query regarding Taxonomy analysis. If I add my desired sequence along with my Greengene database, its not picking my desired sequence in taxanomy analysis. but if i run desired sequence alone mean sequence was Picked up.
For instance, Moraxella; s__osloensis was not seen in sample 2 if i perform taxonomy analysis with greengene database along with desired sequence. it was seen in sample 2 if i perform analysis with desired sequence.
Could anyone tell me how to rectify this error? Herewith, I have attached result and sequence file for your perusal.