Error in qiime2 longitudinal analysis : Pairwise difference comparisons

[Iman (Madhubani Dey)]

Hi all,
I am a new user of qiime2. I am trying to use the qiime2 longitudinal pairwise differences for my data. However, when I am running the code, it is always showing in the output that there are no values for all my samples at all the timepoints that are given in the metadata. Hence, there are no plots and no values for the pairwise difference tests.

My code is:

qiime longitudinal pairwise-differences \
  --m-metadata-file map_FSa98.tsv \
  --m-metadata-file shannon_vector.qza\
  --p-metric shannon_entropy \
  --p-group-column Genotype \
  --p-state-column Timepoint \
  --p-state-1 d00 \
  --p-state-2 d10 \
  --p-individual-id-column Sample \
  --p-replicate-handling random \
  --o-visualization pairwise-differences.qzv

It will be great if anyone can help me with this.

Thanks!

[Keegan-Evans]

@Iman,

Thank you for posting your exact command input, are you getting any error messages? Could you post those and the outputs that you are receiving. Have you inspected your data and metadata to ensure that they do share timepoint values?

[Iman (Madhubani Dey)]

Thank you very much for the reply. I finally got them right, it was a problem with my metadata file.