# Error in qiime picrust2 analysis
**URL:** https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262
**Category:** Library Support
**Created:** [January 27, 2021, 6:02pm UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262 "2021-01-27T18:02:01Z")
**Posts on this page:** 5
**Page:** 1
### Author:  [@divyaprince321](https://forum.qiime2.org/u/divyaprince321)
#### Post date: [January 27, 2021, 6:02pm UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262/1 "2021-01-27T18:02:01Z")
Hello All
I successfully installed qiimepicrust2 after trying so many time, but the whole credit goes to the QIIME team where the members guided me step by step for successful installation.
Thanks.
Now while running the command I faced an unexpected error
"stopping - no asv ids overlap between input fasta and sequence abundance table" at the first time however when I ran at the second time It gave and new error
Simply "Killed"
Please suggest
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### Author:  [@divyaprince321](https://forum.qiime2.org/u/divyaprince321)
#### Post date: [January 27, 2021, 7:23am UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262/2 "2021-01-27T07:23:56Z")
Thanks a lot @ andrewsanchez
I tried couple of times and got the things done.
Thank you for this support
However while running the command
qiime picrust2 full-pipeline --i-table Narwani\_final/Narwanitable.qza --i-seq Narwani\_final/Narwani\_clustered\_sequences.97.qza --output-dir Narwani\_q2-picrust2\_output --p-threads 1 --p-hsp-method pic --p-max-nsti 2 --verbose
Stopping - no ASV ids overlap between input FASTA and sequence abundance table
Please suggest
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### Author:  [@divyaprince321](https://forum.qiime2.org/u/divyaprince321)
#### Post date: [January 27, 2021, 8:00pm UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262/3 "2021-01-27T20:00:46Z")
Hello Again
I opted for the alternative way of the analysis by
# Swapping in SEPP for read placement with q2 picrust2 and ran the first command I got an error
place\_seqs.py -s Narwani\_final/Narwani\_clustered\_sequences/dna-sequences.fasta -o out.tre -p 1 --intermediate intermediate/place\_seqs/epa\_out/epa\_result.jplace
Warning - 41 input sequences aligned poorly to reference sequences (--min\_align option specified a minimum proportion of 0.8 aligning to reference sequences). These input sequences will not be placed and will be excluded from downstream steps.
This is the set of poorly aligned input sequences to be excluded: 836908, 4297918, 109469, 2456449, 250705, 521852, 328281, 208800, 3407058, 4406763, 237889, 779617, 323600, 4348417, 182490, 4469859, 706095, 2432071, 74159, 583766, 131662, 242389, 4396376, 1110769, 737413, 714536, 193866, 774802, 4386673, 4421746, 1124836, 91492, 211578, 4383626, 4446022, 548510, 1119783, 651305, 259220, 712007, 357092
Error running this command:
epa-ng --tree /home/qiime2/miniconda/envs/qiime2-2019.10/lib/python3.6/site-packages/picrust2/default\_files/prokaryotic/pro\_ref/pro\_ref.tre --ref-msa intermediate/place\_seqs/epa\_out/epa\_result.jplace/ref\_seqs\_hmmalign.fasta --query intermediate/place\_seqs/epa\_out/epa\_result.jplace/study\_seqs\_hmmalign.fasta --chunk-size 5000 -T 1 -m /home/qiime2/miniconda/envs/qiime2-2019.10/lib/python3.6/site-packages/picrust2/default\_files/prokaryotic/pro\_ref/pro\_ref.model -w intermediate/place\_seqs/epa\_out/epa\_result.jplace/epa\_out --filter-acc-lwr 0.99 --filter-max 100
Standard output of the above failed command:
INFO Selected: Output dir: intermediate/place\_seqs/epa\_out/epa\_result.jplace/epa\_out/
INFO Selected: Query file: intermediate/place\_seqs/epa\_out/epa\_result.jplace/study\_seqs\_hmmalign.fasta
INFO Selected: Tree file: /home/qiime2/miniconda/envs/qiime2-2019.10/lib/python3.6/site-packages/picrust2/default\_files/prokaryotic/pro\_ref/pro\_ref.tre
INFO Selected: Reference MSA: intermediate/place\_seqs/epa\_out/epa\_result.jplace/ref\_seqs\_hmmalign.fasta
INFO Selected: Filtering by accumulated threshold: 0.99
INFO Selected: Maximum number of placements per query: 100
INFO Selected: Automatic switching of use of per rate scalers
INFO Selected: Preserving the root of the input tree
INFO Selected: Specified model file: /home/qiime2/miniconda/envs/qiime2-2019.10/lib/python3.6/site-packages/picrust2/default\_files/prokaryotic/pro\_ref/pro\_ref.model
INFO Rate heterogeneity: GAMMA (4 cats, mean), alpha: 0.453141 (user), weights&rates: (0.25,0.0250674) (0.25,0.220229) (0.25,0.782933) (0.25,2.97177)
Base frequencies (user): 0.229585 0.22008 0.298596 0.251739
Substitution rates (user): 1.00319 2.79077 1.5301 0.87441 3.83966 1
INFO Selected: Reading queries in chunks of: 5000
INFO Selected: Using threads: 1
INFO \_\_\_\_\_\_ \_\_\_\_ \_\_\_ \_ \_\_ \_\_\_\_\_\_
/ **_// \_\_ \ / | / | / // /
/ \_\_/ / // // /| | \_\_\_\_\_\_ / |/ // / \_\_
/ / / // \_\_\_ |/\_\_\_\_\_// /| // // /
/\_\_\_\_\_/// // || // |_/ \_**\_/ (v0.3.6)
Please suggest
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### Author:  [@divyaprince321](https://forum.qiime2.org/u/divyaprince321)
#### Post date: [January 27, 2021, 9:10pm UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262/4 "2021-01-27T21:10:51Z")
Hello thank you all
I tracked the error in both commands the first one is beause of difference in header ids in biom table and sequences
And the second one is memmory issue.
Bur now my point is how to solve the ist case, I mean to match the ids in biom file similar to that of seq.
If any command please mention.
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [February 28, 2021, 3:10am UTC](https://forum.qiime2.org/t/error-in-qiime-picrust2-analysis/18262/5 "2021-02-28T03:10:52Z")
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