Hello All I am trying to calculate diversity and while given medata file I am getting an error etadata file must be encoded as UTF-8 or ASCII. The following error occurred when decoding the file: 'utf-8' codec can't decode byte 0xa8 in position 10: invalid start byte There may be more errors pr…

Hello @divyaprince321 , first, I suggest that google sheets plugin keemei . I would suggest getting your metadata uploaded to google sheets and using that plugin because it might easily tell you the error. And then you would be good to go. :qiime2: If that fails and you still can't find the solution…

Hi Cherman2 Thank You for response I am not aware of google sheet plugin keemi. I ran the following command qiime diversity core-metrics-phylogenetic --i-phylogeny phylogeny/S1_fast.tree.qza --m-metadata-file metadataS1.qzv --i-table S1_final/clustered_table_cr-97.qza --p-sampling-depth 1 --outp…

Hi @divyaprince321 The original error message is indicating that your metadata file contains invalid characters that QIIME 2 cannot read. This is often caused by creating metadata files in excel, copying and pasting from websites, or sometimes from special characters that are not unicode compatible…

Thanks Chloe I am done with google sheet plugin Keemei, after preparing it when I validated through Keemei and qiime2 it gave a cheering message showing metadata file validated. However again after running the command It gave the error. qiime diversity core-metrics-phylogenetic --i-phylogeny phy…

[image] divyaprince321: --m-metadata-file metadataS1.qzv This seems to be the issue. You need to use your metadata.tsv not the metadata.qzv (A visualization). Hope this helps! Chloe :turtle:

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