# Error in isBimeraDenovoTable(unqs\[\[i\]\], ..., verbose = verbose) : Input must be a valid sequence table.

**URL:** <https://forum.qiime2.org/t/error-in-isbimeradenovotable-unqs-i-verbose-verbose-input-must-be-a-valid-sequence-table/11039>\
**Category:** User Support\
**Tags:** dada2\
**Created:** [August 12, 2019, 12:50pm UTC](https://forum.qiime2.org/t/error-in-isbimeradenovotable-unqs-i-verbose-verbose-input-must-be-a-valid-sequence-table/11039 "2019-08-12T12:50:42Z")\
**Posts on this page:** 1\
**Showing post:** 5

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**Author:** ![kreetelyll](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/k/7feea3/32.png) [@kreetelyll](https://forum.qiime2.org/u/kreetelyll)\
**Post date:** [August 22, 2019, 7:07am UTC](https://forum.qiime2.org/t/error-in-isbimeradenovotable-unqs-i-verbose-verbose-input-must-be-a-valid-sequence-table/11039/5 "2019-08-22T07:07:11Z")

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I am using the same primer pair 515F/806R. I think that I will then just continue on with forward reads, I did a testrun with few samples:  
qiime dada2 denoise-single --i-demultiplexed-seqs import-single-end.qza --p-trunc-len 122 --o-representative-sequences dada2-rep-seqs.qza --o-table dada2-table.qza --o-denoising-stats dada2-stats.qza

and this time it worked and came back without any errors. However I am not entirely sure how to determine where to truncate it exactly. Here I did it at 122 is that okay or what is the best way to decide that? I also read about a method called FIGARO that can help with that. Do you have any knowledge about usage of FIGARO and would recommend using it? [https://www.biorxiv.org/content/10.1101/610394v1](https://www.biorxiv.org/content/10.1101/610394v1)

 ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/8/8855ae94ae6c80adea7570c419d0c2800e9f0338.png)

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