Error in importing FASTQ files

[sakshi (Sakshi Tiwari)]

I am very new to QIIME, and while trying to import files I am facing this error:

manifest.csv is not a(n) PairedEndFastqManifestPhred33 file

Command:
qiime tools import
–type ‘SampleData[PairedEndSequencesWithQuality]’
–source-format PairedEndFastqManifestPhred33
–input-path manifest.csv
–output-path paired-end-demux.qza

[Yos.Dos (Yosef Avrahami)]

Hey,
I dont realy know what is the meaning of your error, but assuming you are using Qiime2 2018.8 your code is wrong try this:

qiime tools import \ --type 'SampleData[PairedEndSequencesWithQuality]' \ --input-path pe-33-manifest \ --output-path paired-end-demux.qza \ --input-format airedEndFastqManifestPhred33

Source: Importing data
Have fun🏁

[Nicholas_Bokulich]

Hi @sakshi,

@sakshi

manifest.csv is not a(n) PairedEndFastqManifestPhred33 file

Invisible characters can cause issues with this file — see these posts and let us know if you are still having trouble (with details on your troubleshooting) after giving those a try. Thanks!