Error-Importing multiplexed fastq

Hi everyone! I have data that contain forward and reverse sequence. So I made a manifest to import.

And then I tried to import this manifest with these code.


However, I got an error that suggested I have no reverse read fastq.

Does anyone help me with solving this error?

MacOS
qiime2-2022.2
anaconda3-2019.7

Hello,

Take a closer look at sample id's - the identical key should tie together forward and reverse read. In your case, different keys (...1.1 and ...1.2) map to forward and reverse files.
Correct the sample-id column.

Cheers
V

2 Likes

Hi,

Thank you for the response! I have successfully imported!

Thank you!
M

1 Like