Hey all,
I am running qiime-2018.11 version and trying to import casava 1.8 demultiplex format. I am re-running the code since I needed to change a couple of labels. I did not have problems with when I run my code the first time (see below):
CarmenP$ qiime tools import
–input-format CasavaOneEightSingleLanePerSampleDirFmt
–input-path fastq/
–output-path SoyExe_demux.qza
–type “SampleData [PairedEndSequencesWithQuality]”
However, now that I want to re-import the data with a couple of changes (I changed the labels of some files from 15 to 15B), I used the following code (the one available in qiime2 docs):
qiime tools import --input-path fastq --input-format CasavaOneEightSingleLanePerSampleDirFmt --output-path demux.qza --type ‘SampleData[PairedEndSequencesWithQuality]’
And I got the following error message:
There was a problem importing fastq:
** fastq is not a(n) CasavaOneEightSingleLanePerSampleDirFmt:**
** Duplicate samples in forward reads: {‘16’}**
This is an example of one of my files names:
1B_45_L001_R1_001.fastq.qza
1B_45_L001_R2_001.fastq.qza
Anyone experienced something similar?
