Error from demux

[Lennon_Lee]

Dear all,

I successfully import my data with PairedEndFastqManifestPhred33:

qiime tools import \
  --type SampleData[PairedEndSequencesWithQuality] \
  --input-path pair-manifest.csv \
  --output-path demux-pair-end.qza \
  --source-format PairedEndFastqManifestPhred33

but when I want to view the demux result I got the problem:

qiime demux summarize \
  --i-data demux-pair-end.qza \
  --o-visualization demux.qzv

Plugin error from demux:

  Error -3 while decompressing data: invalid stored block lengths

Debug info has been saved to /tmp/qiime2-q2cli-err-zg54cf35.log

May I know what am I doing wrong?

Thanks for all the helps and ideas

[thermokarst]

Hey there @Lennon_Lee!

@Lennon_Lee

Error -3 while decompressing data: invalid stored block lengths

Sounds like your source fastq.gz files might be corrupt. Please double-check that you have downloaded all of the files from your sequencing center in full, prior to importing in QIIME 2. Sometimes a center will also give you MD5 sums of the files so that you can ensure their integrity. Hope that helps!

[Lennon_Lee]

Thank you very much!! I re-downloaded the data and solved the problem.