qiime feature-classifier fit-classifier-naive-bayes --i-reference-reads silva132-ref-seqs.qza --i-reference-taxonomy silva132-ref-taxonomy.qza --o-classifier silva132-classifier.qza
After increasing the memory of the VM (35GB) I ran the command like above and got the following error. What is happening? Any comments and solutions are very welcomed!!!
Traceback (most recent call last):
File “/home/qiime2/miniconda/envs/qiime2-2018.4/bin/qiime”, line 11, in
sys.exit(qiime())
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 722, in call
return self.main(*args, **kwargs)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 697, in main
rv = self.invoke(ctx)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 1066, in invoke
return _process_result(sub_ctx.command.invoke(sub_ctx))
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 895, in invoke
return ctx.invoke(self.callback, **ctx.params)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/click/core.py”, line 535, in invoke
return callback(*args, **kwargs)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/q2cli/commands.py”, line 294, in call
path = result.save(output)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/qiime2/sdk/result.py”, line 147, in save
self._archiver.save(filepath)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/qiime2/core/archive/archiver.py”, line 347, in save
self.CURRENT_ARCHIVE.save(self.path, filepath)
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/site-packages/qiime2/core/archive/archiver.py”, line 162, in save
allowZip64=True) as zf:
File “/home/qiime2/miniconda/envs/qiime2-2018.4/lib/python3.5/zipfile.py”, line 1009, in init
self.fp = io.open(file, filemode)
PermissionError: [Errno 1] Operation not permitted: ‘silva132-classifier.qza’