However, this signal below is coming and I am unable to get the output. Can anyone guide me about this error?
/usr/bin/qiime: 52: exec: /usr/lib/qiime/bin/tools.py: not found
This is a QIIME 1 error. Based on that, it looks like you are attempting to run QIIME 2 commands in a QIIME 1 environment. Please see the docs for info on how to install QIIME 2.