Hi @SAHIL_JAIN_16110144 ,qiime tools validate to diagnose the issue, but also check out these posts for nearly identical errors:
I am having some trouble with importing my fastq files for dada2 denoising. I am able to create the "paired-end-demux.qza" file, but get an error message when working with this file. For example, when running "qiime demux summarize" I get the error: " 'utf-8' codec can't decode byte 0xf8 in position 4099: invalid start byte" (full error message is pasted below). I see from the forum that this may have something to do with my manifest file, but I'm out of ideas with what it could be, and my manif…
Hello, I saw there was a similar topic created by somebody but wasn't resolved perhaps. (https://forum.qiime2.org/t/manifest-file-invalid-text-encoding/1827 )
In my case, I am using a demultiplexed fastq file, which I zipped using gzip, and then tried to import using the following command, but couldn't succeed...
qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path ATCC/PGM052_QIIME2.fastq.gz --output-path demux.qza --source-format SingleEndFastqManifestPhred33
Traceback (…