# Epipyxis sp. and Neotessella volvocina detected in groundwater **URL:** https://forum.qiime2.org/t/epipyxis-sp-and-neotessella-volvocina-detected-in-groundwater/29105 **Category:** General Discussion **Tags:** taxonomy **Created:** [January 31, 2024, 10:47am UTC](https://forum.qiime2.org/t/epipyxis-sp-and-neotessella-volvocina-detected-in-groundwater/29105 "2024-01-31T10:47:02Z") **Posts on this page:** 1 **Showing post:** 2
### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer) #### Post date: [January 31, 2024, 10:11pm UTC](https://forum.qiime2.org/t/epipyxis-sp-and-neotessella-volvocina-detected-in-groundwater/29105/2 "2024-01-31T22:11:23Z")
Hi @KQUB, I'd like to point out an important point, as we highlight in our [RESCRIPt tutorial](https://forum.qiime2.org/t/processing-filtering-and-evaluating-the-silva-database-and-other-reference-sequence-data-with-rescript/15494/4), SILVA does not curate species labels, and we warn about this, under the drop menu _"Species-labels: caveat emptor!"_ I'd suggest that you fetch trustworthy sequences of these algal taxa from GenBank and place them into a single FASTA file. Then add some legitimate cyanobacteria too. Then add the sequences of the two potential algal taxa within that same file. From here I'd generate a sequence alignment and a phylogeny. What you should see is two main clades: 1) true cyanobacteria, and 2) chloroplasts. If your sequences land within the chloroplast clade then you can trust that your sequences are indeed chloroplasts and not mis-identified bacteria. You can also submit your potential algal sequences to SILVA's [ACT](https://www.arb-silva.de/aligner/) tool and see if those sequences do actually fall within an algal (chlroroplast) clade. But I'd still be wary about making any over claims with out other data information. --- _[View the full topic](https://forum.qiime2.org/t/epipyxis-sp-and-neotessella-volvocina-detected-in-groundwater/29105)._