# dual barcoding support

**URL:** <https://forum.qiime2.org/t/dual-barcoding-support/19673>\
**Category:** User Support\
**Created:** [May 25, 2021, 5:43am UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673 "2021-05-25T05:43:54Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![liucong2021](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/l/45deac/32.png) [@liucong2021](https://forum.qiime2.org/u/liucong2021)\
**Post date:** [May 25, 2021, 5:43am UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673/1 "2021-05-25T05:43:54Z")

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Was dual-barcode demultiplexing supported in QIIME2 now ???

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**Author:** ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)\
**Post date:** [May 25, 2021, 7:00am UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673/2 "2021-05-25T07:00:47Z")

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Welcome @liucong2021 !

Yes, dual-barcode demultiplexing has been supported in QIIME 2 for over two years now, see the 2019.4 release notes here:

> [@QIIME 2 2019.4 is now available!](https://forum.qiime2.org/t/qiime-2-2019-4-is-now-available/9513):
>
> The QIIME 2 2019.4 release is now available! Thanks to everyone involved for their hard work! As a reminder, our next planned QIIME 2 release is scheduled for late July 2019 (QIIME 2 2019.7), but please stay tuned. Other proposed releases this year include: QIIME 2 2019.7 QIIME 2 2019.10 Check out the [QIIME 2 2019.4 docs](https://docs.qiime2.org/2019.4/) for details on installing the latest QIIME 2 release, as well as tutorials and other resources. Get in touch on the QIIME 2 forum 2 if you run into any issues! Virtual mach…

This is only for unique dual-barcode pairs, not "combinatorial" barcodes (i.e., when the same barcodes are re-used for multiple samples; search the forum archives for more discussion about this and workarounds).

Good luck!

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**Author:** ![liucong2021](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/l/45deac/32.png) [@liucong2021](https://forum.qiime2.org/u/liucong2021)\
**Post date:** [May 25, 2021, 7:30am UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673/3 "2021-05-25T07:30:22Z")

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Thanks. For example, maybe just like,

sample1 A B  
sample2 A C  
sample3 A D  
A、B、C、or D means barcode sequence. Is this kind of unique dual-barcode pairs? Is it supported in newest qiime2 ?

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**Author:** ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)\
**Post date:** [May 25, 2021, 7:31am UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673/4 "2021-05-25T07:31:56Z")

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> [@liucong2021](#):
>
> Is this kind of unique dual-barcode pairs?

No, that scheme is a special case, describes this:

> [@Nicholas\_Bokulich](#):
>
> “combinatorial” barcodes (i.e., when the same barcodes are re-used for multiple samples

unique would be:

sample1 A B  
sample2 C D  
sample3 E F

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**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\
**Post date:** [June 25, 2021, 1:32pm UTC](https://forum.qiime2.org/t/dual-barcoding-support/19673/5 "2021-06-25T13:32:54Z")

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