Hi, professional. I obtained the Demultiplexed file from sequencing company (Fastq.gz). I still a freshman in using QIIME2. Then, I would like to ask some questions in below:
Type: 16S amplicon sequencing rDNA
Primer: V3-V4 region, 341F - 805R
First, I have make a file in .tsv format according to instruction:
Second, I use the join-pairs method to join the reads and generate summary in below:
qiime vsearch join-pairs
--i-demultiplexed-seqs demux.qza \
Third, I use the Deblur method to denoise my sequences, trimming length is about 350. Is it reasonable for this length?
qiime deblur denoise-16S
Fourth, I curious that the calculation and process on table summary. Total frequency is calculated from which value? And, is it reasonable value for number of features (600) in my dataset.
qiime feature-table summarize
Thank you for helping~~