# does q2-itsxpress remove 5.8S from full ITS amplicons?

**URL:** https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096
**Category:** Library Support
**Created:** [August 15, 2019, 8:10am UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096 "2019-08-15T08:10:30Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![sixvable](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/s/439d5e/32.png) [@sixvable](https://forum.qiime2.org/u/sixvable)
#### Post date: [August 15, 2019, 8:10am UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096/1 "2019-08-15T08:10:30Z")

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Wonderful tutorial!  
I just have one question :If I choose region All for my sequences with containing the whole ITS region (from ITS1 to ITS2) ,will 5.8S region still in the trimmed sequences?🤨  
Need your help man

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### Author: ![Adam\_Rivers](https://forum.qiime2.org/user_avatar/forum.qiime2.org/adam_rivers/32/10143_2.png) [@Adam\_Rivers](https://forum.qiime2.org/u/Adam_Rivers)
#### Post date: [August 15, 2019, 12:41pm UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096/2 "2019-08-15T12:41:48Z")

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Yes `all` leaves in the 5.8S.

A word of caution though, typical Illumina 2x300 sequencing is not long enough to span the full ITS region for many fungi. If you use the `all` option for Illumina reads you are likely to have very low rates of merging and very few trimmed reads output by ITSxpress.

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### Author: ![Adam\_Rivers](https://forum.qiime2.org/user_avatar/forum.qiime2.org/adam_rivers/32/10143_2.png) [@Adam\_Rivers](https://forum.qiime2.org/u/Adam_Rivers)
#### Post date: [August 15, 2019, 1:57pm UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096/5 "2019-08-15T13:57:38Z")

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Sorry, I don't understand your issue. Please provide more information if you need help.

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [August 15, 2019, 2:22pm UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096/6 "2019-08-15T14:22:54Z")

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@sixvable - this post is being locked because you are cross-posting, which is disruptive to other participants on the forum. I will follow-up with more details in a private DM to you.

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [August 15, 2019, 3:13pm UTC](https://forum.qiime2.org/t/does-q2-itsxpress-remove-5-8s-from-full-its-amplicons/11096/7 "2019-08-15T15:13:57Z")

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