# Do I need to generate new rep-seqs after filtering?

**URL:** https://forum.qiime2.org/t/do-i-need-to-generate-new-rep-seqs-after-filtering/2297
**Category:** User Support
**Tags:** filtering
**Created:** [December 21, 2017, 1:01am UTC](https://forum.qiime2.org/t/do-i-need-to-generate-new-rep-seqs-after-filtering/2297 "2017-12-21T01:01:23Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![bmillerlab](https://forum.qiime2.org/user_avatar/forum.qiime2.org/bmillerlab/32/674_2.png) [@bmillerlab](https://forum.qiime2.org/u/bmillerlab)
#### Post date: [December 21, 2017, 1:01am UTC](https://forum.qiime2.org/t/do-i-need-to-generate-new-rep-seqs-after-filtering/2297/1 "2017-12-21T01:01:23Z")

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Hello,  
I was able to use qiime2 and successfully denoise my data with dada2. Now I have run several of the filter methods suggested to clean up my asv table. Before I go forward with taxonomy analysis and such am I supposed to redo my rep-seqs file generated by dada2, since I probably removed some of the sequences from the table with the filtering - or does this not matter? If I do need to rerun this, I'm not sure how since the input would not be my demultiplexed sequences anymore?

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### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [December 22, 2017, 6:35pm UTC](https://forum.qiime2.org/t/do-i-need-to-generate-new-rep-seqs-after-filtering/2297/3 "2017-12-22T18:35:20Z")

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Hey @bmillerlab!

It won't really matter, you just may end up annotations for sequences you don't really have in your feature-table anymore. This shouldn't pose any actual problems downstream.

But! If you would like to remove these now redundant sequences, `feature-table filter-seqs` now accepts a feature table in the latest release: [QIIME 2 2017.12](https://forum.qiime2.org/t/qiime-2-2017-12-release-is-now-live/2308)!

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [January 23, 2018, 12:35am UTC](https://forum.qiime2.org/t/do-i-need-to-generate-new-rep-seqs-after-filtering/2297/5 "2018-01-23T00:35:52Z")

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