Hi @Cybele_C,
Here is the key error message:
You changed the sample IDs in your mapping file — that means that they no longer correspond to any of the sample IDs used in any of your data!
Two solutions:
- Just use the original metadata file with the original IDs.
- Use
qiime feature-table group
to relabel your sample IDs in your feature table. See "option 3" in this post: Metadata Index Error - #6 by thermokarst
Good luck!