# Differential abundance analysis: Plugin error from gneiss
**URL:** https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117
**Category:** Technical Support
**Created:** [January 31, 2019, 5:45pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117 "2019-01-31T17:45:15Z")
**Posts on this page:** 7
**Page:** 1
### Author:  [@idapantoja](https://forum.qiime2.org/u/idapantoja)
#### Post date: [January 31, 2019, 5:45pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/1 "2019-01-31T17:45:15Z")
Hi all,
I am trying to run the command:
qiime gneiss ols-regression for the Multivariate response linear regression and I am getting this error message:
Plugin error from gneiss:
[('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance/metadata.yaml', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/metadata.yaml', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/metadata.yaml'"), ('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance/citations.bib', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/citations.bib', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/citations.bib'"), ('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance/action/action.yaml', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/action/action.yaml', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/action/action.yaml'"), ('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance/action', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/action', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/action'"), ('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance/VERSION', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/VERSION', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17/VERSION'"), ('/tmp/qiime2-archive-xusbcxp1/dd9cde43-3230-48a7-a185-a625897d5e17/provenance', '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17', "[Errno 13] Permission denied: '/tmp/qiime2-provenance-1eeg1ybk/artifacts/dd9cde43-3230-48a7-a185-a625897d5e17'")]
Debug info has been saved to /tmp/qiime2-q2cli-err-9y\_g3vnd.log
I am seeing there some "Permission denied". Not sure what that means. I am using version 2018.8. I ran the metadata tabulate to look for errors or blanks and seems to be good. I am attaching it.
[tabulated-sample-metadata.qzv](https://cdck-file-uploads-global.s3.dualstack.us-west-2.amazonaws.com/flex002/uploads/qiime21/original/2X/e/e9e7fd1fdbf56388c12578024150c9de50bb8e22.qzv) (1.2 MB)
This is whole command I am trying to run:
qiime gneiss ols-regression --p-formula "Nat\_SampID+Time+Diet+Date+Time\_Point" --i-table balances.qza --i-tree hierarchy.qza --m-metadata-file mapping\_file\_resistant\_starch\_only65\_differential\_analysis\_dec21.txt --o-visualization regression\_summary.qzv
Thanks for the help!
@idapantoja
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### Author:  [@mortonjt](https://forum.qiime2.org/u/mortonjt)
#### Post date: [January 31, 2019, 6:00pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/2 "2019-01-31T18:00:52Z")
Hi @idapantoja, not exactly sure what is going on here. Could you post the log file /tmp/qiime2-q2cli-err-9y\_g3vnd.log?
It does look like this sort of error has appeared on the qiime2 forums before. Its possible that upgrading to the newest version of qiime2 will do the trick.
> [@q2-ITSxpress: PermissionError: \[Errno 13\] Permission denied: 'classifier.qza'](https://forum.qiime2.org/t/q2-itsxpress-permissionerror-errno-13-permission-denied-classifier-qza/7663):
>
> Hello all, I am trying to train the QIIME classifier as mentioned in the Q2-ITSxpress tutorial. I am using the following command on the server: qiime feature-classifier fit-classifier-naive-bayes \ --i-reference-reads unite.qza --i-reference-taxonomy unite-taxonomy.qza --o-classifier classifier.qza And I am getting the following error: Traceback (most recent call last): File "/opt/qiime2-2018.8/bin/qiime", line 11, in sys.exit(qiime()) File "/opt/qiime2-2018.8/lib/python3.5/site-…
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### Author:  [@idapantoja](https://forum.qiime2.org/u/idapantoja)
#### Post date: [January 31, 2019, 6:38pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/3 "2019-01-31T18:38:30Z")
Hi @mortonjt,
Thanks so much for your quick response!
Attached the file:
[error.txt](https://cdck-file-uploads-global.s3.dualstack.us-west-2.amazonaws.com/flex002/uploads/qiime21/original/2X/f/fd18d52eb1bbc2276dbfeb49bb6609a29fa6f5b2.txt) (2.7 KB)
@idapantoja
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### Author:  [@mortonjt](https://forum.qiime2.org/u/mortonjt)
#### Post date: [January 31, 2019, 7:47pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/4 "2019-01-31T19:47:45Z")
Ok, it looks like you have miniconda running under a root environment
Do you have your own user space? If so, maybe try installing the new version of qiime under your own personal account.
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### Author:  [@idapantoja](https://forum.qiime2.org/u/idapantoja)
#### Post date: [January 31, 2019, 8:00pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/5 "2019-01-31T20:00:49Z")
Hi @mortonjt,
I am using qiime2 in a linux server environment. I can't update qiime2 by myself. That is the only option to be able to run this command?
Thanks!
@idapantoja
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### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 31, 2019, 8:07pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/6 "2019-01-31T20:07:05Z")
Hi @idapantoja --- who installed qiime2 on this server? It is a little unusual that it was installed as the root user --- this is going to cause all kinds of problems with running tools in this environment (not just QIIME 2, but everything in this root miniconda). Maybe you should check in with your sysadmin and see what the story is...
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### Author:  [@mortonjt](https://forum.qiime2.org/u/mortonjt)
#### Post date: [February 7, 2019, 11:23pm UTC](https://forum.qiime2.org/t/differential-abundance-analysis-plugin-error-from-gneiss/8117/7 "2019-02-07T23:23:06Z")
Agree with @thermokarst, its pretty weird if you don't have full control over your environment.
Is it possible for you to install qiime locally? If you were able to run any of the qiime commands (i.e. saving files, moving files, ...), you should be able to install [miniconda](https://conda.io/miniconda.html) to your local directory and then install qiime through conda.