I’m using several methods (i.e. DESeq2, ANCOM, Aldex2) for differential abundance analysis at the ASV level. I am wondering about which of those methods are still suitable (statistically speaking) at higher taxonomic ranks (phylum, class…) when aggregating taxa. Especially, Aldex2 and DESeq2 make distributional assumptions about the ASV counts and I am not sure if it holds true when aggregating taxa.
What would be the most appropriate method or normalization/test association (e.g. CLR + Kruskal-Wallis) to perform such analysis ?