I recently used QIIME2 to run beta diversity analyses on fecal samples. Because we had 4 sequencing runs over a course of a year I used multiple versions of QIIME2(QIIME2-2018 through QIIME2-2019.10) for demultiplexing, filtering and combining feature tables, and running phylogenetic core metrics (labeled as Try1 in screenshots). I recently went back to re-run all analyses in QIIME2 version 2019.10 (labeled as Try2 in screenshots). However, I am getting a very different result in Beta diversity compared to my previous analysis. I have attached photos here of beta diversity results and the provenance files. In try 1, day 25 samples are clustering with the other days. In try 2, day 25 samples are clustering out separately from all other samples. I do not think I did anything differently in the second try, but do you see any major changes that I may have overlooked? Any ideas on why these are so different?