# Didn't use vsearch but its citation is showing up in my provenance

**URL:** https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437
**Category:** User Support
**Tags:** vsearch
**Created:** [October 13, 2022, 10:14pm UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437 "2022-10-13T22:14:14Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![Emily\_Sprague](https://forum.qiime2.org/user_avatar/forum.qiime2.org/emily_sprague/32/13769_2.png) [@Emily\_Sprague](https://forum.qiime2.org/u/Emily_Sprague)
#### Post date: [October 13, 2022, 10:14pm UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437/1 "2022-10-13T22:14:14Z")

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I'm using QIIME 2 v. 2021.11. I used dada2 denoised-paired for quality and the SILVA database with feature-classifier for taxonomy. My taxonomy artifact had the vsearch citation in its provenance. Is that because of the SILVA database, or am I misunderstanding something else?

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [October 14, 2022, 5:27am UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437/2 "2022-10-14T05:27:20Z")

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Hi @Emily_Sprague ,  
You can check the provenance graph to see where this might be introduced.

My guess is that you are using the pre-trained SILVA classifiers or sequences from the QIIME 2 website data-resources. These SILVA-based databases are built using RESCRIPt and include a dereplication step with vsearch (to remove redundant sequences from the database).

This was one motivation behind RESCRIPt: so that the underlying steps used for generating custom reference databases would become visible and hence reproducible by others who want to build their own databases. But I suppose it creates an issue for scraping citations from provenance when re-using previously generated data. So some citation curation may be in order 😁

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### Author: ![Emily\_Sprague](https://forum.qiime2.org/user_avatar/forum.qiime2.org/emily_sprague/32/13769_2.png) [@Emily\_Sprague](https://forum.qiime2.org/u/Emily_Sprague)
#### Post date: [October 15, 2022, 2:53pm UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437/3 "2022-10-15T14:53:01Z")

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I did use the silva-138-99-seqs.qza file, so that makes sense. Thanks for explaining it. Another thought for citation curation: my artifacts generated later in the workflow had two or three duplicates for some of the citations, so when I downloaded the Bibtex files into my reference manager I had to do some deleting.

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [October 18, 2022, 5:26am UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437/4 "2022-10-18T05:26:20Z")

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Hi @Emily_Sprague ,

> [@Emily\_Sprague](#):
>
> Another thought for citation curation: my artifacts generated later in the workflow had two or three duplicates for some of the citations, so when I downloaded the Bibtex files into my reference manager I had to do some deleting.

For sure — this is because some underlying packages are key parts of multiple plugins or actions, so when parsing all citations in a provenance tree you get duplicate entries and need to dereplicate.

This plugin might be of interest/use to you here. `provenance-replay` has a `replay citations` action (mentioned but not shown in this tutorial?) to parse all citations in the provenance tree and dereplicate these:

> [@Provenance Replay Beta Release and Tutorial](https://forum.qiime2.org/t/provenance-replay-alpha-release-and-tutorial/23279):
>
> Hello everyone! I'm super excited to announce the alpha release (update: beta release) of provenance-lib, software I've been building to support scientific reproducibility, attribution, and collaboration on the QIIME 2 platform. tada :qiime2: tada [provenance\_lib is available in alpha release on the QIIME 2 Library](https://library.qiime2.org/plugins/provenance_lib/43/)tada :qiime2: tada Update: As of QIIME 2 2023.9, provenance-lib has been integrated into QIIME 2. For your entertainment and edification, I've included examples of some fun…

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [November 18, 2022, 11:26am UTC](https://forum.qiime2.org/t/didnt-use-vsearch-but-its-citation-is-showing-up-in-my-provenance/24437/5 "2022-11-18T11:26:37Z")

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