Denoising with DADA2 - Single runs vs. Pooled

Hi @Mike_Stevenson,

There has been lots of discussion on which approach to use, and there really isn't a great solution to pooling or not, you may have to change other parameters during pooling, and/or sequencing depth as discussed here.

I personally like to use --p-pooling-method pseudo --p-chimera-method pooled along with --p-min-fold-parent-over-abundance 8, or 16, but not higher, as mentioned here.

Does this help?

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