Hi @Mike_Stevenson,
There has been lots of discussion on which approach to use, and there really isn't a great solution to pooling or not, you may have to change other parameters during pooling, and/or sequencing depth as discussed here.
I personally like to use --p-pooling-method pseudo --p-chimera-method pooled
along with --p-min-fold-parent-over-abundance 8
, or 16
, but not higher, as mentioned here.
Does this help?