I noticed that for the previous error, the file name that is referenced (T2163_12S_CGCAGTCTAT_L002_R1_001.fastq.gz) is not even the file name that I see, but it is a file name that appears in my sequencing report as the demultiplexed file name (sampleID_index_lane#_read#_001.fastq). I guess the demultiplexing process somehow deciphers that from the header? I removed that T2163 file and this time got the follow error with qiime tools validate:
Traceback (most recent call last):
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/site-packages/q2cli/builtin/tools.py", line 409, in validate
result = qiime2.sdk.Result.load(path)
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/sdk/result.py", line 66, in load
archiver = archive.Archiver.load(filepath)
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/core/archive/archiver.py", line 305, in load
rec = archive.mount(path)
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/core/archive/archiver.py", line 204, in mount
root = self.extract(filepath)
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/site-packages/qiime2/core/archive/archiver.py", line 215, in extract
zf.extract(name, path=str(filepath))
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/zipfile.py", line 1507, in extract
return self._extract_member(member, path, pwd)
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/zipfile.py", line 1577, in _extract_member
with self.open(member, pwd=pwd) as source,
File "/opt/conda/envs/qiime2-2019.10/lib/python3.6/zipfile.py", line 1393, in open
raise BadZipFile("Truncated file header")
zipfile.BadZipFile: Truncated file header
There was a problem loading /data-store/iplant/home/wlandesman/RO3-Plate4/12S-Trimmed/demux-trimmed-rem2163-redo.qza as a QIIME 2 Result:
Truncated file header
See above for debug info.