Hai running QIIME2.2019.4 with demultiplex paired end sequence
I have import the file using
qiime tools import
–output-path demux-paired-end.qza, because no barcode is avilable
Then convert the demux-paired-end .qza to demux-paired-end.qzv
qiime demux summarize --i-data demux-paired-end.qza --o-visualization demux.qzv
It shows error like
Plugin error from demux:
Compressed file ended before the end-of-stream marker was reached
Debug info has been saved to /tmp/qiime2-q2cli-err-35003ux7.log
Can any help in this issue
Thank you for your valuable time.
Could you share your log file?
Preliminarily, it looks like there might be an issue with the compression of one of your files. Are they gzipped? What happens if you try to gunzip one?
Thanks for your reply
All the files are gzipped.
No error if I import 4-5 samples, and works fine, but more than 15 file (forward and reverse fastq. gz) qiime2 shows the error.
That suggests there’s an issue with a specific sample (or subset of samples). Your log file might help figure out which samples. Alternatively, you could try seeing if you exclude single samples where the failure happens and then work from there.
Thanks for your quick reply.
How to get the log file, help me in this issue.
You would find this file on your computer and copy the contents here.
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