# Demultiplexing multiple marker genes with overlapping barcodes

**URL:** https://forum.qiime2.org/t/demultiplexing-multiple-marker-genes-with-overlapping-barcodes/1451
**Category:** User Support
**Tags:** demux
**Created:** [October 9, 2017, 4:36pm UTC](https://forum.qiime2.org/t/demultiplexing-multiple-marker-genes-with-overlapping-barcodes/1451 "2017-10-09T16:36:47Z")
**Posts on this page:** 1
**Showing post:** 5

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### Author: ![jairideout](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jairideout/32/9_2.png) [@jairideout](https://forum.qiime2.org/u/jairideout)
#### Post date: [October 10, 2017, 12:04am UTC](https://forum.qiime2.org/t/demultiplexing-multiple-marker-genes-with-overlapping-barcodes/1451/5 "2017-10-10T00:04:35Z")

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Just noticed this is a duplicate topic. Locking this topic and moving further support here:

> [@Multi-gene amplicon sequences with dada2](https://forum.qiime2.org/t/multi-gene-amplicon-sequences-with-dada2/1402):
>
> Hello, We amplified four gene markers (16S, 18S, 23S, rbcL) for each sample and combined all four markers before adding the barcode and sequencing. I now have demultiplexed sequences but each sample still contains sequences of all four amplicons. Do I need to create individual files for each sample and marker before processing with dada2? Each amplicon is a different size but I figure I can use the primer to parse them out. Is there anything in place to assist me with this? Thanks, Danny

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