Demultiplexing error2

Hello everyone!
I have an error when performing multiplexing,

(qiime2-amplicon-2023.9) constanzamendez@MacBook-Air-de-Constanza raw_seqs % qiime demux emp-paired
--m-barcodes-file sample-metadata.tsv
--m-barcodes-column barcode-sequence
--p-rev-comp-mapping-barcodes
--i-seqs emp-paired-end-sequences.qza
--o-per-sample-sequences demux-full.qza
--o-error-correction-details demux-details.qza
Plugin error from demux:

Barcodes of different lengths were detected: 21 != 17. Variable length barcodes are not supported.

Debug info has been saved to /var/folders/wf/q2n_rd_930j8_ykq42vcllz40000gn/T/qiime2-q2cli-err-ysq3svj8.log

I'm using manifest files and I don't know if I'm using the correct configuration.

I appreciate your help,

Hello @constanza_mendez_vej. Your barcode sequences should be in one column of the sample-metadata.tsv you passed into the --m-barcodes-file argument.. All barcode sequences must be the same length, but it sounds like yours are not. I'm not sure how to handle this, but I'll ask around.

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Hello @constanza_mendez_vej. Can you DM me your sample-metadata.tsv?

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