Apart from the dialogue above, if I have .txt file extension instead of .tsv format in metadata file, Will Qiime2 work for demultiplexing?
By the way, I put this command:
qiime demux emp-paired \
I got this error:
There was an issue with loading the file Barcodemetadate.txt as metadata:
Metadata file path doesn’t exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory): Barcodemetadate.txt
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2019.1/tutorials/metadata/
I have already checked my metadata file with Keemei. It sent me ‘Good Job’! i did not have any error on Keemei!
What is the path problem?
I checked this discussion. I did not get any help!