I am trying to demultiplex raw data downloaded as seqs.fastq.qz (preprocessed fastq) from Qiita. I followed the "Moving Picture" tutorial which required me to import the data first.
Because my data was in “EMP protocol” multiplexed paired-end fastq format, I was directed to "Importing data" help page and it says that I need three files: forward.fastq.gz, reverse,fastq.gz, barcodes.fastq.gz. However, it seems like, in my case, the barcode, forward sequences and reverse sequences are all in one seq.fastq.gz file. I wonder how I could separate them into three individual file?
Thank you very much~