I am having some issues during the demultiplex stage:
qiime demux emp-single
Q1- Is there any method to demultiplex without associate to barcode (In Qiime 1.9.0 I used the multiple_split_libraries_fastq.py –i folderpairedsequence –o outputfolder –demultiplexing-method sampleid-by-file? Is there any similar command like this one in QIIME 2?
I have a folder with the paired files and another with the Mapping File (Metadata).
How can I do the demultiplex without the metadata and barcode files, like this command in QIIME 1.9.2 multiple_split_libraries_fastq.py?
I would greatly appreciate for any clarification.