# Demuliplex-sequences still contan primers, how can you run DADA2?

**URL:** <https://forum.qiime2.org/t/demuliplex-sequences-still-contan-primers-how-can-you-run-dada2/4475>\
**Category:** User Support\
**Tags:** dada2\
**Created:** [June 6, 2018, 1:02pm UTC](https://forum.qiime2.org/t/demuliplex-sequences-still-contan-primers-how-can-you-run-dada2/4475 "2018-06-06T13:02:37Z")\
**Posts on this page:** 1\
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**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\
**Post date:** [June 6, 2018, 1:10pm UTC](https://forum.qiime2.org/t/demuliplex-sequences-still-contan-primers-how-can-you-run-dada2/4475/3 "2018-06-06T13:10:28Z")

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Hi @Cheng50373640!

> [@Cheng50373640](#):
>
> How can you run DADA2 after import demuliplexed sequences?

The q2-dada2 methods actually only accept demultiplexed sequences as input, so this is perfect!

> [@Cheng50373640](#):
>
> because they still contain primers or/and barcodes.

You can use the q2-cutadapt plugin to remove primers or barcodes. Check out this [community tutorial](https://forum.qiime2.org/t/demultiplexing-and-trimming-adapters-from-reads-with-q2-cutadapt/2313) for examples.

Hope that helps! 🦖 :qiime2:

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