Hi,
Anyone knows how can i know the degrees of freedom from PERMANOVA?
Thanks
Hi,
Anyone knows how can i know the degrees of freedom from PERMANOVA?
Thanks
Two ways:
qiime diversity adonis
, which will report Df in the visualizationbeta-group-significance
you can calculate Df manually. I believe for this test Df = n - 1 where N = the number of unique groups (for categorical variables) or Df = 1 (for numeric variables) but you should check the original article to confirm.Hi, thank you for your reply.
The Df from adonis is the same from the permanova?
And, what do you mean with beta-diversity? I did permanova with bray curtis matrix.
Beta diversity is the difference / distance between samples. So the distance matrix of distances between samples shows the beta diversity of those samples.
(Alpha diversity is the richness and/or evenness of each individual sample, while beta diversity is between samples.)
Colin
To add to @colinbrislawn’s point, your Bray-Curtis matrix is a beta-diversity matrix. Bray Curtis is one measure of beta-diversity.
Yes i know but, i did permanova with bray curtis matrix so, i got a little confused when @Nicholas_Bokulich gave me those two options.
So, what i want to know is how can i determine the Df from Permanova (that was done with bry curtis matrix)?
Thank you
I answered this question above. You say you did permanova with a bray curtis matrix, so if you did this in QIIME 2 you must have used one of the two methods that I described above. Even if you did not do this in QIIME 2, then my answer above still answers your question. Here's the answer:
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