# Deblur plugin error **URL:** \ **Category:** User Support\ **Tags:** deblur\ **Created:** [December 11, 2017, 5:47pm UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172 "2017-12-11T17:47:57Z")\ **Posts on this page:** 5\ **Page:** 1
**Author:** ![Sausage\_Mahoney](https://forum.qiime2.org/user_avatar/forum.qiime2.org/sausage_mahoney/32/361_2.png) [@Sausage\_Mahoney](https://forum.qiime2.org/u/Sausage_Mahoney)\ **Post date:** [December 11, 2017, 5:47pm UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172/1 "2017-12-11T17:47:57Z")
Hi all- Getting the following plugin error from deblur: ![image](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/1X/c562870eb161c72895b287db77ebf3195701ea15.jpg) Error log below: Traceback (most recent call last): File "/home/qiime2/miniconda/envs/qiime2-2017.11/bin/deblur", line 4, in **import** ('pkg\_resources').run\_script('deblur==1.0.3', 'deblur') File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/pkg\_resources/ **init**.py", line 750, in run\_script self.require(requires)[0].run\_script(script\_name, ns) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/pkg\_resources/ **init**.py", line 1524, in run\_script exec(code, namespace, namespace) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/deblur-1.0.3-py3.5.egg-info/scripts/deblur", line 684, in deblur\_cmds() File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/click/core.py", line 722, in **call** return self.main(\*args, \*\*kwargs) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/click/core.py", line 697, in main rv = self.invoke(ctx) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/click/core.py", line 1066, in invoke return \_process\_result(sub\_ctx.command.invoke(sub\_ctx)) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/click/core.py", line 895, in invoke return ctx.invoke(self.callback, \*\*ctx.params) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/click/core.py", line 535, in invoke return callback(\*args, \*\*kwargs) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/deblur-1.0.3-py3.5.egg-info/scripts/deblur", line 632, in workflow threads\_per\_sample=threads\_per\_sample) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/deblur/workflow.py", line 879, in launch\_workflow error\_dist, indel\_prob, indel\_max) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/deblur/deblurring.py", line 114, in deblur seqs = get\_sequences(input\_seqs) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/deblur/deblurring.py", line 65, in get\_sequences ", ".join(map(str, unaligned\_lengths)))) ValueError: Not all sequence have the same length. Aligned lengths: 288, sequence lengths: 250, 251 Traceback (most recent call last): File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/q2cli/commands.py", line 218, in **call** results = action(\*\*arguments) File "", line 2, in denoise\_16S File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/qiime2/sdk/action.py", line 220, in bound\_callable output\_types, provenance) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/qiime2/sdk/action.py", line 355, in _callable\_executor_ output\_views = self.\_callable(\*\*view\_args) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/q2\_deblur/\_denoise.py", line 96, in denoise\_16S hashed\_feature\_ids=hashed\_feature\_ids) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/site-packages/q2\_deblur/\_denoise.py", line 163, in \_denoise\_helper subprocess.run(cmd, check=True) File "/home/qiime2/miniconda/envs/qiime2-2017.11/lib/python3.5/subprocess.py", line 398, in run output=stdout, stderr=stderr) subprocess.CalledProcessError: Command '['deblur', 'workflow', '--seqs-fp', '/tmp/q2-SingleLanePerSampleSingleEndFastqDirFmt-tviopad3', '--output-dir', '/tmp/tmpoeslway1', '--mean-error', '0.005', '--indel-prob', '0.01', '--indel-max', '3', '--trim-length', '-1', '--min-reads', '10', '--min-size', '2', '--jobs-to-start', '1', '-w', '--keep-tmp-files']' returned non-zero exit status 1 The .qza file is the output from the following script: $qiime tools import --type 'SampleData[PairedEndSequencesWithQuality]' --input-path /home/qiime2/Desktop/Scowden/Fecal\_analysis/ --source-format CasavaOneEightSingleLanePerSampleDirFmt --output-path Fecal-demux-paired-end.qza Thanks S ---
**Author:** ![antgonza](https://forum.qiime2.org/user_avatar/forum.qiime2.org/antgonza/32/1236_2.png) [@antgonza](https://forum.qiime2.org/u/antgonza)\ **Post date:** [December 11, 2017, 8:54pm UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172/3 "2017-12-11T20:54:47Z")
Hi @Sausage_Mahoney, Deblur operates only on same length sequences. In other words, all sequences should be the same length. Anyway, by default `--p-trim-length` is set to -1 so no trimming actually happens in the deblur pipeline. Suggest setting this value to your desired length. Based on the error: `Aligned lengths: 288, sequence lengths: 250, 251`, perhaps 250 is a good value. Best, ---
**Author:** ![Sausage\_Mahoney](https://forum.qiime2.org/user_avatar/forum.qiime2.org/sausage_mahoney/32/361_2.png) [@Sausage\_Mahoney](https://forum.qiime2.org/u/Sausage_Mahoney)\ **Post date:** [December 11, 2017, 9:21pm UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172/5 "2017-12-11T21:21:32Z")
Hi @antgonza! Cripes...totally forgot about that. I have successfully run the script in the past...but couldn't figure out what the problem was this time. Thanks S ---
**Author:** ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)\ **Post date:** [December 11, 2017, 9:56pm UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172/6 "2017-12-11T21:56:41Z")
An off-topic reply has been split into a new topic: [Empty deblur stats viz](https://forum.qiime2.org/t/empty-deblur-stats-viz/2175) Please keep replies on-topic in the future. ---
**Author:** ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)\ **Post date:** [January 12, 2018, 3:56am UTC](https://forum.qiime2.org/t/deblur-plugin-error/2172/7 "2018-01-12T03:56:54Z")
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