Deblur Plugin Error-quality control

User Support
1

When I was running deblur for the 16s V3-V4 data, I encountered this error:

qiime deblur denoise-16S \ 
Traceback (most recent call last):
  File "/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/bin/qiime", line 7, in <module>
    from q2cli.__main__ import qiime
  File "/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/__main__.py", line 11, in <module>
    import q2cli.commands
  File "/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/commands.py", line 15, in <module>
    import q2cli.tools
  File "/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/tools.py", line 344, in <module>
    default=os.getcwd())
FileNotFoundError: [Errno 2] No such file or directory
(qiime2-2018.8) [huangshi@gnode8 qiime2]$   --i-demultiplexed-seqs demux-joined-filtered20.qza \
>   --p-trim-length 150 \
>   --p-sample-stats \
>   --p-jobs-to-start 10 \
>   --o-representative-sequences rep-seqs150.qza \
>   --o-table table150.qza \
>   --o-stats deblur-stats150.qza
-bash: --i-demultiplexed-seqs: command not found

Thanks so much!

2

The version of qiime2 is qiime2-2018.8. Running data2, there was the same plugin error.Traceback (most recent call last):
File “/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/bin/qiime”, line 7, in
from q2cli.main import qiime
File “/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/main.py”, line 11, in
import q2cli.commands
File “/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/commands.py”, line 15, in
import q2cli.tools
File “/home/gene/huangshi/miniconda3/envs/qiime2-2018.8/lib/python3.5/site-packages/q2cli/tools.py”, line 344, in
default=os.getcwd())
FileNotFoundError: [Errno 2] No such file or directory

3

Hey there @tengfey!

It looks like something is going wrong when copying and pasting - please try writing the command out instead and see if that works for you.

If it doesn’t, can you provide some more info about your QIIME 2 deployment?

4

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