Data importing problem

Hi all, I'm quite new with QIIME 2 :relieved:, I wanted to import data from fastq files and create qza files, I used the following command

qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path /mnt/c/Bioinfo/manifest.tsv --output-path /mnt/c/Bioinfo/sequences.qza --input-format SingleEndFastqManifestPhred33V2

However, I got an error :roll_eyes::
/mnt/c/Bioinfo/manifest.tsv is not a(n) SingleEndFastqManifestPhred33V2 file

Herein, I attached the manifest file.
I'm not sure where is the problem exactly.
I appreciate your help :heavy_heart_exclamation:
manifest.tsv (575 Bytes)

Hi @sayed,

Welcome to the :qiime2: forum!

The file you've attached uses commas (,) to seperate the data, technically making it a csv (comma-sepeerated-value) file... despite the extension you've used.
You need to use tabs, which are usually represented as \t. If you're using a spreadsheet programing (excel/google docs etc), the tsv option may be labeled .txt instead but will probably include "tab" in the name. If you're using R/python, when you save the file, use \t as your seperator.

Best,
Justine

2 Likes

thank you so much for your reply.
I tried to separate the tabs and avoid using the commas, herein the file after edit. Can you please confirm me whether it's fine or not ?
Thanks a lot :pray: :pray: :pray:

manifest.tsv (8.8 KB)

Hi @sayed,

I'm going to go back to. my earlier recommendations: if you're preparing this in a spreadsheet program, save it as text, if you're preparting programatically, use '\t' as your sep value.

Best,
Justine

1 Like

Ok, thanks for your kindness. I appreciate your help.

1 Like

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