# DADA2 to Qiime2 **URL:** https://forum.qiime2.org/t/dada2-to-qiime2/26764 **Category:** User Support **Created:** [June 13, 2023, 7:08am UTC](https://forum.qiime2.org/t/dada2-to-qiime2/26764 "2023-06-13T07:08:17Z") **Posts on this page:** 1 **Showing post:** 2
### Author: ![Keegan-Evans](https://forum.qiime2.org/user_avatar/forum.qiime2.org/keegan-evans/32/10422_2.png) [@Keegan-Evans](https://forum.qiime2.org/u/Keegan-Evans) #### Post date: [June 14, 2023, 12:45am UTC](https://forum.qiime2.org/t/dada2-to-qiime2/26764/2 "2023-06-14T00:45:25Z")
@kulandai1, unfortunately there is no way to perform individual DADA2 steps in the QIIME 2 implementation. It might be possible to do something like this: - import individual denoised samples as `SampleData[Sequences]` - dereplicate them - merge the resulting individual `FeatureTable[Frequency]` and `FeatureData[Sequence]` artifacts using [merge](https://docs.qiime2.org/2023.5/plugins/available/feature-table/merge/) and [merge-seqs](https://docs.qiime2.org/2023.5/plugins/available/feature-table/merge-seqs/) - [remove chimeras](https://docs.qiime2.org/2023.5/plugins/available/vsearch/uchime-denovo/) using vsearch - cluster and continue on with the analysis But this is certainly a non-standard workflow, and it might be helpful to take a look at the [clustering otus](https://docs.qiime2.org/2023.5/tutorials/otu-clustering/) and the [filtering chimeric feature sequences](https://docs.qiime2.org/2023.5/tutorials/chimera/) tutorials for inspiration. Another difficulty might be importing the RDS data into QIIME 2, though [this older community tutorial](https://forum.qiime2.org/t/importing-dada2-and-phyloseq-objects-to-qiime-2/4683) might be helpful here. I can't say that I am overly optimistic about this working, but if you can't simply start by pulling all of the data into QIIME 2 initially, this might let you move forward! The easiest solution may be to merge the RDS files in R using a machine with more than 76 GB of RAM, though I am not familiar enough with that operation to know how much more you would need. --- _[View the full topic](https://forum.qiime2.org/t/dada2-to-qiime2/26764)._