# DADA2- Samples from Multiple MiSeq Sequencing Runs

**URL:** https://forum.qiime2.org/t/dada2-samples-from-multiple-miseq-sequencing-runs/1311
**Category:** User Support
**Tags:** dada2, merge
**Created:** [September 25, 2017, 6:06pm UTC](https://forum.qiime2.org/t/dada2-samples-from-multiple-miseq-sequencing-runs/1311 "2017-09-25T18:06:39Z")
**Posts on this page:** 1
**Showing post:** 4

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### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [September 25, 2017, 11:27pm UTC](https://forum.qiime2.org/t/dada2-samples-from-multiple-miseq-sequencing-runs/1311/4 "2017-09-25T23:27:28Z")

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Hi @Shalabh_Suman,

> [@Shalabh\_Suman](#):
>
> Means if it’s possible to analyze samples together that are from multiple MiSeq runs and push them through the QIIME2/DADA2 or would we need to analyze samples from one run at a time and then put the OTU/feature-table results together in the end?

To expand on @Sirtaj-Singh's answer a bit, you probably shouldn't mix multiple runs.  
DADA2 is meant to operate on a single run at a time (it builds an error model for each run, so mixing runs will just confuse the model and the way it picks out the ASVs).

Once you are familiar with the moving pictures tutorial, you should check out [this one](https://docs.qiime2.org/2017.8/tutorials/fmt/), which specifically deals with managing multiple runs.

Let me know if you have any other questions about this!

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