DADA2 Return Code -11 Error when running metagenomic sequences

[Rainjie]

Hi all!

I'm trying to process metagenomic sequences derived from ENA human gut metagenome with qiime2 in order to best compare them with my own 16S amplicon data.

The following error message occurs:

An error was encountered while running DADA2 in R (return code -11), please inspect stdout and stderr to learn more.

So,I went to the forum to search for possible solutions, someone said 'q2-dada2 is not designed to handle shotgun data' and i should use either 'q2-shogun' or 'q2-metaphlan2' for metagenomic analyze.

But I found that others have offered another solution. In short, it is to increase the 'operating memory'.

Here is my question:Can i use qiime for metagenomic data analysis and How should I do?

Here is my error log.
R version 4.0.2 (2020-06-22)
Loading required package: Rcpp
DADA2: 1.18.0 / Rcpp: 1.0.5 / RcppParallel: 5.0.2

1. Filtering ...................................
2. Learning Error Rates
   7320948624 total bases in 50839921 reads from 1 samples will be used for learning the error rates.

*** caught segfault ***
address 0x2b7d68af7000, cause 'memory not mapped'

[jwdebelius (Justine Debelius)]

Hi @Rainjie,

@Rainjie

Here is my question:Can i use qiime for metagenomic data analysis and How should I do?

I think you're looking at two issues. The post you linked is correct: DADA2 is not designed for metagenomic data and is not the correct tool. The plugins you linked are more appropriate for processing metagenomic sequences.

Best,
Justine

[Rainjie]

Many thanks! I will try.