# Dada2 return code 1 error (5/2020)

**URL:** https://forum.qiime2.org/t/dada2-return-code-1-error-5-2020/14774
**Category:** Technical Support
**Tags:** dada2
**Created:** [May 4, 2020, 4:14pm UTC](https://forum.qiime2.org/t/dada2-return-code-1-error-5-2020/14774 "2020-05-04T16:14:22Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![ukuzma2](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ukuzma2/32/7997_2.png) [@ukuzma2](https://forum.qiime2.org/u/ukuzma2)
#### Post date: [May 4, 2020, 4:14pm UTC](https://forum.qiime2.org/t/dada2-return-code-1-error-5-2020/14774/1 "2020-05-04T16:14:22Z")

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Hello,

I'm trying to analyze a cutadapt trimmed .fastq file (150 bp single end reads on an Illumina MiSeq) with Qiime2 and am running into an error with Dada2. I've tried searching for the same error but I haven't seen others with the same .log output.

I'm running qiime2-2020.2 which was conda installed natively.

I successfully imported my “Fastq manifest” format sequence as a SingleEndFastqManifestPhred33V2 running this command:

qiime tools import   
--type 'SampleData[SequencesWithQuality]'   
--input-path P\_putida\_S16\_gDNA\_Library\_V6\_Manifest   
--output-path V6\_Input\_Fragments\_Trimmed.qza   
--input-format SingleEndFastqManifestPhred33V2

[V6\_Input\_Fragments\_Trimmed.qzv](https://forum.qiime2.org/uploads/short-url/kyHEnxVpFc0cIt91Oef8P6sMwQA.qzv) (268.7 KB)

If I'm not mistaken the sample was already demultiplexed coming from Illumina BaseSpace (in Casava 1.8 format) so I went straight to Dada2.

I ran this command:

qiime dada2 denoise-single   
--i-demultiplexed-seqs V6\_Input\_Fragments\_Trimmed.qza   
--p-trim-left 0   
--p-trunc-len 150   
--o-representative-sequences rep-seqs-dada2.qza   
--o-table table-dada2.qza   
--o-denoising-stats stats-dada2.qza

However, I get an error with Dada2 (return code 1).

[200502 Dada2 Error.txt](https://forum.qiime2.org/uploads/short-url/hGDtQqrrOhrfCkca8uEw9b9RRs4.txt) (2.5 KB)

As I understood it, there is an input error?

Thank you for your help!

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [May 4, 2020, 7:58pm UTC](https://forum.qiime2.org/t/dada2-return-code-1-error-5-2020/14774/4 "2020-05-04T19:58:35Z")

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Hi @ukuzma2, glad to hear you got things working - rather than deleting the post, how about we keep the post (and your solution) around, so that future forum users can learn from this? Thanks!

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [June 5, 2020, 1:58am UTC](https://forum.qiime2.org/t/dada2-return-code-1-error-5-2020/14774/5 "2020-06-05T01:58:35Z")

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