# Dada2 Parameter problem

**URL:** https://forum.qiime2.org/t/dada2-parameter-problem/17384
**Category:** User Support
**Created:** [November 9, 2020, 11:44am UTC](https://forum.qiime2.org/t/dada2-parameter-problem/17384 "2020-11-09T11:44:12Z")
**Posts on this page:** 1
**Showing post:** 3

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### Author: ![andrewsanchez](https://forum.qiime2.org/user_avatar/forum.qiime2.org/andrewsanchez/32/20160_2.png) [@andrewsanchez](https://forum.qiime2.org/u/andrewsanchez)
#### Post date: [November 9, 2020, 7:30pm UTC](https://forum.qiime2.org/t/dada2-parameter-problem/17384/3 "2020-11-09T19:30:43Z")

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Please see my response to your question in [Remove Primer in paired-end demultiplexed file](https://forum.qiime2.org/t/remove-primer-in-paired-end-demultiplexed-file/17376). **It looks like you didn't actually remove the primers** , so I'm not so sure it would make sense to run dada2 on data that still contain non-biological sequences.

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