# DADA2 : low non chimeric read counts after denoising step , --p-min-fold-parent-over-abundance

**URL:** https://forum.qiime2.org/t/dada2-low-non-chimeric-read-counts-after-denoising-step-p-min-fold-parent-over-abundance/32625
**Category:** Technical Support
**Tags:** dada2
**Created:** [February 25, 2025, 2:27pm UTC](https://forum.qiime2.org/t/dada2-low-non-chimeric-read-counts-after-denoising-step-p-min-fold-parent-over-abundance/32625 "2025-02-25T14:27:07Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [February 25, 2025, 2:35pm UTC](https://forum.qiime2.org/t/dada2-low-non-chimeric-read-counts-after-denoising-step-p-min-fold-parent-over-abundance/32625/2 "2025-02-25T14:35:53Z")

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> [@Sue](#):
>
> I’ve read various posts suggesting that adjusting `--p-min-fold-parent-over-abundance` might help, but I’m not sure what value to use.

HI @Sue, the suggested values are outlined in this [post](https://forum.qiime2.org/t/trimming-output-problem/29631/14), along with references. I'd suggest not going above 16 if possible.

To help I'd also lower the truncation values too... by default DADA2 requires at least 12 bp of overlap. I'd suggest reading [this post](https://forum.qiime2.org/t/questions-about-v3-v4-primers-for-16s-rrna-amplicon-sequencing-and-calculating-overlap/20250) for more detail.

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