Hi timanix,
I checked other forum post, if I run it with 230 for both sides, I think I will have 4bp gap, right?
(forward read) + (reverse read) - (length of amplicon) - = overlap
230+230-464 = -4 bp,
then my reads cannot merge at all because they don't overlap.
Or, should I discard truncation and lower the value of overlap region which may save the reads loosing on merging step?
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