I am attempting to trim sequences from 16 different samples in the qiime2-2017.10 local environment and I keep getting this error message: Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. Debug info has been saved to /var/folders/xs/mzb2ccb925z5vs7t4ws_8lzm0000gn/T/qiime2-q2cli-err-ffivvwzk.log
When I inspect the file saved to the directory, it simply says “0, C, Cleanup at Startup, T”. My code that I entered to get this error looks like this:
qiime dada2 denoise-paired --i-demultiplexed-seqs /Users/loxia19/Desktop/demux.qza --o-table /Users/loxia19/Desktop/table-dada2 --o-representative-sequences /Users/loxia19/Desktop/rep-seqs-dada2.qza --p-trim-left-f 10 --p-trim-left-r 5 --p-chimera-method consensus --p-trunc-q 30 --p-trunc-len-f 220 --p-trunc-len-r 200 --p-n-threads 40 --p-n-reads-learn 20000
I have tried running this code with the bare minimum arguments (just --o-representative-sequences, --p-trunc-left-f and --p-trunc-left-f). I updated to the latest R software even though I’m not using it, and I tried running the command with the --verbose argument attached. Nothing has worked and I am continually getting the same error! If anyone knows how to solve this issue, feedback would be greatly appreciated!!