Hey guys,
I'm getting the following error when running DADA2.
Fontconfig error: Cannot load default config file
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-dbuh42rc.log
The source data is Illumina.
To import I ran :
qiime tools import \
--type 'SampleData[PairedEndSequencesWithQuality]' \
--input-path manifest_19_20.csv \
--output-path paired-end-demux-qza \
--input-format PairedEndFastqManifestPhred33
The manifest content :
sample-id,absolute-filepath,direction
sample-19,$HOME/cnete/dataset/MI.M03992_0107.001.BioOHT_19rc.SCI017689_PCR2-19-CNETE_R1.fastq.gz,forward
sample-19,$HOME/cnete/dataset/MI.M03992_0107.001.BioOHT_19rc.SCI017689_PCR2-19-CNETE_R2.fastq.gz,reverse
sample-20,$HOME/cnete/dataset/MI.M03992_0107.001.BioOHT_20rc.SCI017689_PCR2-20-CNETE_R1.fastq.gz,forward
sample-20,$HOME/cnete/dataset/MI.M03992_0107.001.BioOHT_20rc.SCI017689_PCR2-20-CNETE_R2.fastq.gz,reverse
I ran this DADA2 command :
qiime dada2 denoise-paired
--i-demultiplexed-seqs paired-end-demux-s19_20.qza
--p-trim-left-f 0
--p-trunc-len-f 245
--p-trim-left-r 0
--p-trunc-len-r 186
--p-max-ee 2
--p-n-threads 64
--o-representative-sequences rep-seqs-dada2-all.qza
--o-table table-dada2-all.qza
--o-denoising-stats stats-dada2-all.qza
The error file is attached to this message (3.2 KB). It seems to be related to isBimeraDenovoTable function.
Please note, my knowledge in bio is near to null.
Other file : paired-end-demux-19_20.qzv (291.3 KB)
Thanks for helping!
