DADA2 denoise-paired error

I am running QIIME2 2018.2. I ran the following command
qiime dada2 denoise-paired
–i-demultiplexed-seqs ~/Riley/AXRS/Demux/demux.qza
–p-trim-left-f 21
–p-trunc-len-f 205
–p-trim-left-r 21
–p-trunc-len-r 206
–o-representative-sequences ~/Riley/AXRS/DADA2/rep-seqs-dada2.qza
–o-table ~/Riley/AXRS/DADA2/table-dada2.qza
–output-dir ~/Riley/AXRS/DADA2

And received this error:

  1. Filtering Error in validObject(.Object) :
    invalid class “SRFilterResult” object: superclass “Mnumeric” not defined in the environment of the object’s class
    Execution halted
    Plugin error from dada2:

An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.

Could you please assist? I don’t know how to interpret this error.

Thank you!

Hi @rlhughes,

I’ve never seen that before. My first hunch is that something is interacting with the QIIME 2 environment that shouldn’t be. We recently figured out how to prevent that from happening in QIIME 2 2018.4, so maybe try installing the newest version.

Otherwise, I would have to ping @benjjneb on this.

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Sorry, just got around to trying this but seems to be working now that I updated to 2018.4! Thank you

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